X-ZELL Cryoimmunostaining™ SUITE

The world's first slide-based, 9-colour immunostaining solution

X-ZELL’s Cryoimmunostaining™ Suite is a new, ultra-precise platform solution designed to make next-generation single-cell immunofluorescence cytology accessible to researchers worldwide.

Originally designed to enable X-ZELL’s own research into next-generation biomarkers for the early detection of clinically significant prostate cancer, it is suited for visualizing and characterising membrane, cytosolic and nuclear antigen expression in single cells as well as organ tissue.

With the ability to avoid crosstalk, background and non-specific binding, it is the only multiplexed immunostaining system on the market that does not require spectral unmixing. 

Perfected as part of X-ZELL’s own laboratory routine, it therefore allows researchers to analyse tissue and blood samples at a whole new level of detail while still using standard antibodies.

Currently validated for 18 commonly used flowcytometry fluorophores* and their spectral equivalents, it covers the entire light spectrum for the visualization and characterisation of membrane, cytosolic and nuclear antigen expression in single cells as well as organ tissue – all with minimal crosstalk between fluorescence channels and no downstream application of advanced mathematical algorithms.

Proven in immunophenotyping, cell-cell interactions, immunostaining, rare-cell research, slide-based cytometry and many more applications, the X-ZELL Cryoimmunostaining™ Suite will give you next-generation performance now – and at 50-80% less cost.

*incl. DAPI, BV421, Pacific Blue, Pacific Orange, BV480, PerCp, AF488, PE, PE-Cy5, PE-Cy7, Propidium Iodide, AF594, DRAQ5, AF647, AF750, AF790, AF800, DIR

X-ZELL CryoFixator™


  • Rapid, gentle, non- toxic cell fixation on standard slides
  • Retain cell morphology & tissue architecture
  • Recover >90% of rare cells on standard microscope slides
  • 50-70% RNA preservation
  • 80- 100% DNA preservation
  • Process 8 slides per run
  • Footprint: 402mm x 579mm x 139mm(LxWxH)
  • Run Time: 35 min
  • Hands-on Time: 2 min


X-ZELL CryoStainer™

  • Single-step, 9-color immunofluorescence staining
  • Membrane, cytosolic and nuclear antigens
  • Optimised for suspended cells & cryo-sections 
  • Samples fully re-stainable 
  • Low background & non-specific antibody binding
  • High resolution images without spectral unmixing
  • Process 8 slides per run
  • 50-80% less antibody usage 
  • Footprint: 402mm x 579mm x 139mm(LxWxH)
  • Run Time: 1h 45 min
  • Hands-on Time: 5 min



start discovering

To unleash the full potential of X-ZELL’s world-unique Cryoimmunostaining™ Suite, users require access to a fully automated fluorescence microscope with X-ZELL fluorescence filter sets.

Contact sales@x-zell.com for more information on microscope set-up and detailed operational work flows, as well as to re-order reagents and X-ZELL CapGap equipment™.

To obtain detailed Cryoimmunostaining™ process protocols, please contact info@x-zell.com.

X-ZELL Publications

Bhakdi et al. Accuracy of Tumour-associated Circulating Endothelial Cells as a Screening Biomarker for Clinically Significant Prostate Cancer. Cancers, 2019

Bhakdi et al. Navigating the Diagnostic Grey zone with tCEC (Poster). Asia-Pacific Prostate Cancer Conference, 2018

Bhakdi et al. Navigating the Diagnostic Grey zone with tCEC (Abstract). BJU International, 2018

Bhakdi et al. Easy Employment and Crosstalk-Free Detection of Seven Fluorophores in a Widefield Fluorescence Microscope. Methods & Protocols, 2018

Waseem et al. Buffer-Optimized High Gradient Magnetic Separation: Target Cell Capture Efficiency is Predicted by Linear Bead-Capture Theory. Journal of Magnetics, 2016

Waseem et al. Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding. International Journal of Molecular Sciences, 2014

Bhakdi et al. Optimized High-Gradient Magnetic Separation for Isolation of Plasmodium-infected Red Blood Cells. Malaria Journal, 2010



© 2020 X-ZELL INC

Find Us