X-ZELL Cryoimmunostaining™ SUITE

slide-based multiplexing with up to 9 fluorophores simultaneously

SPIN-OFF PRODUCT: X-ZELL CRYOIMMUNOSTAINING™ SUITE SLIDE-BASED MULTIPLEXING WITH UP TO 9 FLUOROPHORES SIMULTANEOUSLY

  • UP TO 9 FLUOROPHORES PER SLIDE
  • VALIDATED FOR 18 COMMON FLUOROPHORES*
  • SIMPLE PROTOCOL FOR EASY LAB INTEGRATION
  • COMPATIBLE WITH EXISTING MICROSCOPES**
X-ZELL’s Cryoimmunostaining™ Suite is part of a novel, patented platform technology designed by X-ZELL to enable the identification of novel biomarkers in whole blood.

The system has been tested for 18 different fluorophores*, up to nine of which may be utilised in parallel to enable the fast and direct visualisation and characterization of membrane, cytosolic and nuclear antigen expression in single cells as well as frozen organ tissue. It is compatible with the patented X-ZELL CapGap™ slide holding system, which will reduce antibody usage by 50-80%.

To ensure minimal crosstalk between fluorescence channels and avoid complex and time-consuming downstream processing such as spectral unmixing, X-ZELL has developed a series of Reagent & Consumable Kits optimized for use with the
Cryoimmunostaining™ system (available separately).

The result is a powerful, slide-based multiplexing solution that does not affect cell morphology or tissue architecture and will keep both RNA and DNA content intact for follow-up analysis.

The X-ZELL Cryoimmunostaining™ Suite is currently available in select markets as a spin-off product. Please contact X-ZELL for more information.

*The X-ZELL Cryoimmunostaining™ Suite has been tested for the following fluorophores and their spectral equivalents: DAPI, BV421, Pacific Blue, Pacific Orange, BV480, PerCp, AF488, PE, PE-Cy5, PE-Cy7, Propidium Iodide, AF594, DRAQ5, AF647, AF750, AF790, AF800, DIR
Note: X-ZELL laboratory instruments have been validated for use in combination with official X-ZELL consumable & reagent kits. Third-party reagents and consumables have not been validated and may affect instrument performance. Unless specifically indicated, all X-ZELL products are designated as general laboratory equipment.

X-ZELL Publications

Bhakdi et al. Immunocytomorphological landscape of tCAC in the blood of lung cancer patients (abstract, ECC 2021) Advances in Clinical and Experimental Medicine 2021

Narula et al. Slide-based immunocytometry for diagnostics of acute leukemia (abstract, Blood 2021) Blood 2021 Abstract Book 2021

Bhakdi et al. Improved analysis of tumour-associated circulating rare cells (abstract, ECP 2021) Virchows Archiv 2021

Chiangjong et. al. Cell-main spectra profile screening technique in simulation of CTC using MALDI-TOF Cancers 2021

Bhakdi et al. Cytomorphologic and immunophenotypic characterization of tCAC in prostate adenocarcinoma (abstract, ECP 2020) Virchows Archiv 2020

Bhakdi et al. Accuracy of tCEC as a screening biomarker for clinically significant prostate cancer Cancers 2019

Bhakdi & Thaicharoen Easy employment and crosstalk-free detection of seven fluorophores in a widefield fluorescence microscope Methods & Protocols 2018

Bhakdi Tumour-derived circulating endothelial cells predict clinically significant prostate carcinoma (abstract, APCC 2018) BJU International 2018

Waseem et al. Buffer-optimized high-gradient magnetic separation for isolation of plasmodium-infected RBC Journal of Magnetics 2016

Waseem et al. Antibody-conjugated paramagnetic nanobeads: Kinetics of bead-cell binding International Journal of Molecular Sciences 2014

Bhakdi et al. Optimized high-gradient magnetic separation for isolation of plasmodium-infected RBC Malaria Journal 2010

X-ZELL CryoFixator™

 

  • Rapid, gentle, non- toxic cell fixation on standard slides
  • Validated for suspended cells & cryo-sections
  • Full retention of cell morphology & tissue architecture
  • >90% rare cell recovery on standard microscope slides
  • 50-70% RNA preservation
  • 80- 100% DNA preservation
  • Capacity: 8 slides per run
  • Footprint: 421mm x 581mm x 159mm (LxWxH)
  • Hands-on Time: 2 min

X-ZELL CryoStainer™

  • Custom panels with up to 9 fluorophores per experiment
  • 50-80% less fluorophore consumption 
  • Validated for 18 common fluorophores*
  • Samples fully re-stainable 
  • No crosstalk
  • No autofluorescence
  • No non-specific antibody binding
  • No spectral unmixing
  • Capacity: 8 slides per run
  • Footprint: 421mm x 581mm x 159mm (LxWxH)
  • Hands-on Time: 5 min

X-ZELL

FIGHTING CANCER,
ONE CELL AT A TIME.

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