Application & Resources
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more data per sample [5]
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less time from sample to digital slide [5]
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fewer touch points per sample [5]
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less hands-on time per sample [5]
THE MULTIPLEXING EFFECT
X-ZELL’s Next-Generation Cytology (NGC) platform is the result of a decade of interdisciplinary R&D in multiplex immunostaining, digital pathology and diagnostic workflow integration. First presented at the 2023 European Congress of Cytology in Budapest, Hungary, NGC has since attracted early adopters in both Europe and Southeast Asia and was the subject of multiple clinical studies.
IMPLEMENTATION
NGC is designed to integrate smoothly into existing laboratory environments with minimal disruption.
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Standardized protocols and intuitive software interfaces allow rapid adoption by technical staff.
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Direct-to-slide fixation compatible with all common sample preparation methods without requiring cell-blocking.
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Fully digital slide files that can be reviewed locally or shared for remote consultation.
UPSTREAM COMPATIBILITY
The NGC platform is optimized for native effusion & FNA samples and is compatible with all common monolayer preparation methods incl. ThinPrep®, Cytospin™, smears, frozen sections & imprints [2,5,7]
LATEST PUBLICATIONS
Miceska S, Kholová I, Kloboves Prevodnik V. Multiplex Cryoimmunostaining in Fine-Needle Aspiration Biopsies And Effusion Samples: Preliminary Results. Acta Cytologica 2026.
Bhakdi SC, Fives C, Grote S, Battmann A. Multiplexed Effusion Immunodiagnostics in Routine Cytopathology (Abstract, ICC25). Acta Cytologica 2025.
Miceska S, Bhakdi SC, Kloboves Prevodnik V. Multiplex Immunocytology of Fine-Needle Aspiration Biopsies and Effusions: Initial Findings (Abstract, ICC25). Acta Cytologica 2025.
Miceska S, Bhakdi SC, Kloboves Prevodnik V. Multiplex Cryoimmunostaining Platform: Application to Standardly Prepared Cytospin Samples in a Routine Cytopathology Laboratory to Avoid Wastage (Abstract, ECP25) Virchows Archiv 2025.
Battmann A. Cryoimmunostaining – A New Method in Cytological Diagnostics of Effusions (Proffered Paper, ECC24).
GLOSSARY
Cryoimmunostaining™: Novel immunostaining method utilising the X-ZELL Cryoimmunostaining™ Suite to simultaneously apply up to eight diagnostic antibodies on a single microscope slide to maximise diagnostic yield and sample utilization.
Cryoimmunostaining™ Suite: Patented laboratory solution including the X-ZELL Cryofixator™ and X-ZELL Cryostainer™.
Cryofixator™: Patented laboratory instrument enabling non-toxic direct-to-slide sample fixation in a stable temperature environment without requiring cell-blocking. Part of the Cryoimmunostaining™ Suite. CE-MARKED.
Cryostainer™: Patented laboratory instrument enabling slide-based immunofluorescence staining with up to eight fluorophore-conjugated primary antibodies on a single microscope slide. Part of the Cryoimmunostaining™ Suite. CE-MARKED.
Cryophore™: New X-ZELL product line of proprietary antibody-dye conjugates designed for use with the NGC platform.
Hybrid Microscope: Proprietary workstation consisting of a semi-automated fluorescence microscope and corresponding software to enable the digitisation of multiplexed microscope slides in >30s. CE-MARKED.
Next-Generation Cytology (NGC): Umbrella term for the application of new and innovative solutions developed by X-ZELL, including the X-ZELL Cryoimmunostaining™ Suite and X-ZELL Hybrid Microscope, to maximize diagnostic yield from non-gynaecological cytologic sample material.
Next-Generation Cytology (NGC) platform: End-to-end laboratory solution combining the X-ZELL Cryoimmunostaining™ Suite, Hybrid Microscope and all relevant reagents and consumables to enable digital cytopathology in a clinical routine environment.
Multiplexed immunostaining / multiplexing: Simultaneous application of multiple diagnostic antibodies on a single microscope slide to maximize diagnostic yield and sample utilization.
Footnotes
[1] The X-ZELL Cryofixator™ and Cryostainer™ are classified as general laboratory equipment (IVDR Class A / US FDA Class I). The Hybrid Microscope software is classified as Software as a Medical Device (SaMD) under EU MDR 2017/745. Unless otherwise specified, X-ZELL reagents and consumables are intended for research use or general laboratory applications.
[2] X-ZELL internal data. More information upon request.
[3] X-ZELL laboratory instruments have been validated for use in combination with official X-ZELL consumables and reagents. Third-party reagents and consumables have not been validated and may affect instrument performance.
[4] Hybrid Microscope: USFDA Reg. No.: 864.36, EU ID: G580XZELLHybrScopeAFS; Cryoimmunostaining™ Suite: USFDA Reg. No.: 862.205, EU ID: G580XZELLCryofixatorBXK/ G580XZELLCryostainerB3R.
[5] Publications pending. More information upon request.
[6] Aisner DL, Sams SB. The role of cytology specimens in molecular testing of solid tumors: Techniques, limitations, and opportunities. Diagnostic Cytopathology [Internet]. 2012 May 22;40(6):511–24. Available from: https://doi.org/10.1002/dc.22820
[7] 22nd International Congress of Cytology 2025, May 11-18, 2025, Florence, Italy: Abstracts. Acta Cytologica [Internet]. 2025 May 9;69(Suppl. 1):1–94. Available from: https://doi.org/10.1159/000545256
[8] Miceska et al, Multiplex Cryoimmunostaining in Fine-Needle Aspiration Biopsies an Effusion Samples: Preliminary Results. Acta Cytologica. 2026. doi:10.1159/000550736.
